model：81156 81151 80050
specifications ：60 pieces/box
introduction：a 35 mm imaging dish with a polymer coverslip bottom for high-end microscopy and cell-based assays in this microscopy dish, the cells are imaged on a no. 1.5 ibidi polymer coverslip bottom with the highest optical quality and optimal cell adherence
cultivation and high-resolution imaging of cells
widefield and confocal fluorescence microscopy of living and fixed cells
live cell imaging over extended time periods
differential interference contrast (dic) when using a dic lid
cell location and counting when using the gridded version
|ø µ-dish||35 mm|
|growth area||3.5 cm2|
|coating area using 400 µl||4.1 cm2|
|ø observation area||21 mm|
|height with / without lid||14/12 mm|
standard format imaging dish with a 35 mm diameter for tissue culture
ibitreat (tissue culture-treated) surface for optimal cell adhesion and cell growth
high walls with a standard height for easy handling; low wall version available: μ-dish 35 mm, low
lid with locking feature for minimal evaporation
rim for easy opening
biocompatible plastic material—no glue, no leaking
available as a bulk pack with 400 individually packed μ-dishes per box
available with a #1.5h glass coverslip bottom: μ-dish 35 mm, high glass bottom, specifically suitable for tirf and single molecule applications
available with a 500 μm grid for cell counting: μ-dish 35 mm, high grid-500
available with a non-adhesive bioinert surface: μ-dish 35 mm, high bioinert, specifically suitable for 3d applications, such as spheroids and suspension cells
the principle of the μ-dish 35 mm, high
the coverslip bottom
the μ-dish 35 mm, high comes with a thin ibidi polymer coverslip bottom that has the highest optical quality (comparable to glass) and is ideally suitable for high-resolution microscopy. it is also available with a #1.5h glass coverslip bottom for tirf or single molecule microscopy.
as another option for cost-effective high-throughput experiments, we offer the glass bottom dish 35 mm with a #1.5 glass coverslip bottom.
the ibitreat surface
ibitreat (tissue culture-treated) is our most recommended surface modification, because almost all adherent cells grow well on it without the need for any additional coating.
lid with locking feature for minimized evaporation
all ibidi μ-dishes are equipped with the special lid-locking feature. the locking position minimizes evaporation, and thereby provides excellent conditions for long-term studies in a non-humidified environment. gas exchange (carbon dioxide or oxygen) during cell culture is maintained thanks to the gas-permeable plastic material of the dish.
tip: use the locking feature if minimal evaporation is required (e.g., outside incubators, non-humidified microscopy stages, etc.).
ibidi µ-dish height variations
µ-dish 35 mm, high
high walls for all standard cell culture applications
μ-dish 35 mm, low
low walls that provide greater cell access, which is useful for micromanipulation
triple immunofluorescence of bovine endothelial cells.
red: mitochondria, stained with mitotracker? red cmxros;
green: f-actin, stained with alexa fluor? 488 phalloidin;
blue: nuclei, stained with dapi.
comparison of the ibidi dishes 35 mm
please find a detailed comparison of the material specifications, including suitable microscopy applications for the ibidi polymer coverslip, the ibidi glass bottom, and further materials here.