µ-slide i luer glass bottom

model:80167 80177 80187 80197



introduction:channel slides with a #1.5h glass bottom for flow applications; different heights and volumes available

adherent cells under flow conditions

cell culture (static or stop-flow)

simulation of blood vessels with endothelial cells

high-resolution microscopy of living and fixed cells

tirf and super-resolution microscopy

want to know if you should use a glass or a polymer bottom for your application? find out here.

because of the adhesive layer, the channel height of the µ-slide i luer glass bottom increases by 50 µm compared to the µ-slide i luer.


outer dimensions

25.5 x 75.5 mm² (w x l)

channel length

50 mm

channel width

5 mm


female luer

volume per reservoir

60 μl

growth area

2.5 cm2

bottom: glass coverslip no. 1.5h, selected quality, 170 µm /- 5 µm

µ-slide i luer glass bottom





channel height

250 µm

450 µm

650 µm

850 µm

channel volume

62.5 µl

112.5 µl

162.5 µl

212.5 µl

coating area

5.2 cm²

5.4 cm²

5.6 cm²

5.8 cm²

*standard format with thin coverslip bottom made from d 263 m schott glass #1.5h (170 µm /- 5 µm) for low or high magnification microscopy

*large observation area for microscopy

*defined shear stress and shear rate levels

*easy connection to tubes and pumps using luer adapters

*fully compatible with the ibidi pump system

*may require coating to promote cell attachment

*also available as a µ-slide i luer with an ibidi polymer coverslip bottom for superior cell growth

the principle of the µ-slide i luer glass bottom

the glass bottom

the µ-slide i luer glass bottom comes with a thin #1.5h glass coverslip bottom that has the highest optical quality and is ideally suitable for high-resolution microscopy, tirf, and super-resolution microscopy. to promote cell attachment, a surface coating might be required prior to cell seeding.

find more information and technical details about the coverslip bottom of the ibidi chambers here.

choosing the right channel height for flow applications

which experiment are you planning?

low channels are more suitable for flow applications.

high channels are more suitable for static cell culture.

for flow assays with small amounts of medium and high values of shear stress

0.2 channel

for a wide range of shear stress

0.4 channel

for controlling low values of shear stress < 2 dyne/cm2

0.6 and 0.8 channels

select the ideal perfusion set for your flow application here.

cross section of the different channel heights

* not recommended for static culture over more than 6 hours

experimental example

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